Using SSR with fluorescent labels to establish SSR molecular identity cards for cultivars of tree peony Paeonia rockii
To distinguish and protect new cultivars of Paeonia rockii effectively, we developed molecular identity cards for 154 cultivars of P. rockii selected by ourselves. Thirty-three pairs of SSR fluorescent primers were used to amplify all samples in all 154 cultivars. GenAlEx 6.503 and Microsatellite Toolkit were used to calculate the genetic parameters of every primer. By the rule to identify the most cultivars with the least pairs of primers, we found that the combination of the 12 pairs of primers, such as PS095, PS356, PS166, PS119, PS271, PS068, PS367, PS074, PS157, PS221, PS159 and PS187, can very effectively distinguish all 154 cultivars, so that we use these 12 pairs of primers to have successfully establish the molecular identity cards for every cultivar. This study provides an accurate and effective molecular detection method for the identification of P. rockii cultivars. It is also of great significance for the registration, utilization and protection of new tree peony cultivars.
Guo, X., Cheng, F.Y., Cheng, X.Y. and Tao, X.W. (2020). Using SSR with fluorescent labels to establish SSR molecular identity cards for cultivars of tree peony Paeonia rockii. Acta Hortic. 1283, 131-136
SSR markers, molecular identity cards, tree peony (Paeonia rockii), cultivar protection