Characterization of the PEBP protein family in the genus Rosa

F. Foucher, D. Lakwani, A. Chastellier, T. Thouroude, J. Clotault
The PEBP (phosphatydil ethanolamine-binding protein) family is a small protein family in plants that plays a key role in adaptation of plants to environment. PEBPs were discovered as important regulators of flowering with FT, a major component of the florigen signal and TFL1 a floral repressor that also controls inflorescence architecture. In rose, a TFL1 homolog, RoKSN, is controlling continuous flowering whereas RoFT, the FT homolog was proposed to control the date of blooming. In this study, our objective was to analyze in detail the different members of the PEBP family in the genus Rosa. Using the new released high-quality rose genome sequences, we performed a manual annotation of the genes encoding PEBP family members. Using re-sequencing data from seven wild rose species, we have analyzed the PEBP-encoding genes. In the reference rose haploid genome, we have detected 3 clades of encoded proteins. The FT/TSF clade contains two proteins in rose and three in Fragaria vesca. The MFT clade has one member. The TFL1 clade contains 4 proteins: RoATC and RoKSN, the TFL1/ATC homologs, and two BFT homologs. Concerning these two homologs, one (RC6G0343500) is orthologous with the F. vesca member, the other has no ortholog. The corresponding gene (RC1G0537200) has no intron, whereas all other genes encoding the PEBP family have three introns. Globally, a similar organization was found in the 7 re-sequenced genomes. Concerning the BFT homolog in the TFL1 clade, the no-intron homolog was not found in R. persica, an ancestral species in the Rosa genus. This suggests that the origin of this duplication (through RNA recombination) occurred early in rose speciation. Using the reference sequence and new re-sequenced genomes, we were able to characterize in detail the PEBF family in rose and propose hypotheses concerning the evolution of this family within the Rosa genus.
Foucher, F., Lakwani, D., Chastellier, A., Thouroude, T. and Clotault, J. (2020). Characterization of the PEBP protein family in the genus Rosa. Acta Hortic. 1283, 181-190
DOI: 10.17660/ActaHortic.2020.1283.25
phosphatydil ethanolamine-binding protein, duplication, rose evolution

Acta Horticulturae