Influence of media composition and genotype for successful Cannabis sativa L. in vitro introduction
In vitro plant propagation allows to obtain in several crops a higher reproduction rate than the classic vegetative propagation methods, and also it permits to reduce the surface of the area designated for producing the mother plants and for rooting the cuttings. Also, in vitro rooted clones can be more easily transported and shipped abroad due to its sterility. One of the most limiting factors to tackle successfully the first in vitro introduction of a new cultivar is the different response to the addition of different sources of basal salts mixtures, vitamins, carbon, plant growth regulators, and gelling agents. The traditional and most used medium in plant tissue culture is the well-known Murashige and Skoog (MS) medium, consisting in a mixture of basal salts and vitamins. In our experiments, media were prepared by using, or not, Gamborg (B5) mixture of vitamins, and supplemented with 3% sucrose, 0.8% agar and at pH adjusted to 5.8. Two different combinations of phytohormones were used: meta-topoline vs. naphthaleneacetic acid + indole-3-butyric acid. Multiwell plates were incubated under an 18-h photoperiod at 25°C for a week, and continually monitored. The aim of the investigation was to find the best media for in vitro introduction and culture of six different cultivars of Cannabis sativa L. MS was compared to Formula β, an alternative basal salts mixture that has produced significant better results in previous studies on this crop. Axillary buds were harvested from young branches of a donor mother plant. Buds of each cultivar were plated onto MS basal salts medium, as well as onto a Formula β and onto a modified version of Formula β, defined as A. The percentage of survival and germinated shoot tips was recorded, and results indicated that the success was cultivar-dependent.
Codesido, V., Meyer, S. and Casano, S. (2020). Influence of media composition and genotype for successful Cannabis sativa L. in vitro introduction. Acta Hortic. 1285, 75-80
Murashige and Skoog, Formula β, vitamins, axillary buds, plant growth regulators