In vitro propagation and preservation of promising chrysanthemum cultivars and hybrid forms
Chrysanthemum (Chrysanthemum × morifolium Ramat.) is one of the most popular flowering ornamental plants cultivated all over the world. Biotechnological approaches are actively used for propagation and preservation of valuable ornamental cultivars. For our investigation, 0.25-0.3 mm meristems and shoot nodal segments (1.0 cm) were used as primary explants. The in vitro morphogenetic capacity of chrysanthemum explants was studied. Explants cultured on MS medium with 0.75-1.0 mg L‑1 BAP and 0.25 mg L‑1 NAA resulted in formation of hyperhydrated microshoots and development of non-morphogenic callus at their base. The MS medium with 0.4-0.75 mg L‑1 kinetin, 2.5-5.0 mg L‑1 adenine sulfate and 20.0 g L‑1 sucrose promoted the growth of well-developed adventitious buds and microshoots. For the long-term in vitro preservation of viable chrysanthemum explants, optimal concentrations of CCC (chlorocholine chloride) were found out. The presence of CCC promoted inhibition in the growth of chrysanthemum explants compared to the control. Screening of studied cultivars and forms under the effect of different low positive temperatures and CCC within 10 months made it possible to select the ones with a high viability. Thus, at CCC concentration of 0.2-0.4 g L‑1 the amount of viable explants in chrysanthemum was 90-100%. Explants transferred to the standard culture conditions formed adventitious microshoots and developed plantlets. It was noted that, after preservation, the propagation rate in the studied chrysanthemum cultivars and hybrid forms increased 2-3 times.
Mitrofanova, O.V., Lesnikova-Sedoshenko, N.P., Ivanova, N.N., Smykova, N.V. and Mitrofanova, I.V. (2020). In vitro propagation and preservation of promising chrysanthemum cultivars and hybrid forms. Acta Hortic. 1285, 139-146
Chrysanthemum × morifolium, conservation, explant, in vitro, morphogenetic capacity, regeneration