Micropropagation of selected almond (Amygdalus communis L.) genotypes

B. Şan, A.N. Yıldırım, F. Yıldırım, B. Bayar, Y. Karakurt
In the study, the possibility of micropropagation of 42 selected almond genotypes was investigated. For this purpose, shoot tips taken in April were subjected to surface disinfection and then cultured in the MS medium containing 1 mg L‑1 BAP and 0.1 mg L‑1 IBA. The number of shoots explant‑1, shoot length and vitrification occurrence were determined in the cultures growing in a climate chamber for 6 subcultures. In the 42 genotypes tested, the total number of shoots explant‑1 varied between 1.75 and 3.81. The number of shoots longer than 1 cm ranged between 0.97 and 2.30 shoots explant‑1, and the number of shoots smaller than 1 cm ranged from 0.75 to 2.04 shoots explant‑1. Vitrification, which occurred in significant proportions in some genotypes, negatively affected the possibilities of micropropagate them. At the end of the shoot propagation stage, healthy and high-quality shoots of the best 14 genotypes were rooted on the MS medium containing half-strength macroelements, 1.0 mg L‑1 IBA and 120 mg L‑1 sequestrene as iron source. The rooting percentages of the 14 genotypes ranged between 3.3 and 37.0%, with values higher than 25% for genotypes 9, 29 and 134.
Şan, B., Yıldırım, A.N., Yıldırım, F., Bayar, B. and Karakurt, Y. (2020). Micropropagation of selected almond (Amygdalus communis L.) genotypes. Acta Hortic. 1285, 167-172
DOI: 10.17660/ActaHortic.2020.1285.26
propagation, in vitro, rooting, plant growth regulators, vitrification

Acta Horticulturae