SETIS™, a novel variant within the temporary immersion bioreactors
The temporary immersion system SETIS consists of an upper recipient for in vitro plants and a connected lower recipient for liquid medium. The combination of periodical short immersion with nutrients and regular aeration of the headspace allows fast in vitro growth of a large number of plant species. Benefits and drawbacks of the system are discussed and points of attention and pitfalls during set-up and maintenance are shared. To illustrate that temporary immersion system requires its own medium optimization, a culture of pear in SETIS was compared with a classical culture on semi-solid medium.
Lotfi, M. and Werbrouck, S.P.O. (2020). SETIS™, a novel variant within the temporary immersion bioreactors. Acta Hortic. 1285, 253-258
micropropagation, mass production, in vitro, TIS