White cabbage microspore response to low temperature treatment as a trigger for embryogenesis

T.O. Cristea, S. Ambarus, M. Calin, C. Brezeanu, P.M. Brezeanu
In Brassica species obtaining doubled haploid plants is a key tool for the production of commercial F1 hybrids. Among the many methods employed for this purpose, the culture of isolated microspores offers the opportunity to generate double haploid embryos starting from single haploid cells, thus assuring the genetic purity of haploid plants obtained. Embryogenesis and plant regeneration, the ultimate goals for microspore culture, can be achieved by the application of different stress treatments that were proved, by the studies, to potentiate the shift between normal gametic evolution of microspore toward the sporophitic one. Despite the multiple studies accomplished, the response of microspores is still not well elucidates, as there are many factors that are involved in this process. The stresses used to determine the sporophytic development of the microspores can be categorized in many ways, the most important ones being according to the time of application (before or during the culture), or type of stress (cold, heat, starvation), etc. The present study is focused in the determination of cold pre-treatments effect applied to buds for 4 to 7 days prior to isolation. The control variant is represented by microspores isolated from freshly cut buds. The results obtained offer an integrative perspective over the effect of cold pre-treatment over the microspore evolution, providing a strong base for future cultivation protocols for white cabbage.
Cristea, T.O., Ambarus, S., Calin, M., Brezeanu, C. and Brezeanu, P.M. (2020). White cabbage microspore response to low temperature treatment as a trigger for embryogenesis. Acta Hortic. 1285, 281-285
DOI: 10.17660/ActaHortic.2020.1285.40
embryogenesis, stress, pollen, Brassica, species

Acta Horticulturae