Molecular characterization of ten Heliconia (Heliconiaceae) genotypes by means of RAPD markers

M. Malakar, P. Acharyya, S. Biswas
Heliconia a trendiest specialty cut flower of tropics is gaining popularity for its inflorescence’s charismatic form and alluringly blended hues in the world and in Indian market too. It is a monophyletic genus with roughly 200 to 250 species of neotropical origin. It resides in the Heliconiaceae family of Zingiberales order former incorporated in Musaceae for fusion of inverted flowers, single staminode and drupe type fruits elite for it. Exploitation of molecular markers may aid in perceptive recognition of several species and cultivars, since taxonomic confusions and uncertainties subsist among them. In this context, ten species and cultivars were analyzed using RAPD markers. Thirty primers among 70 were chossen, which amplified 1281 polymorphic DNA fragments with each primer giving a mean of 42.7 polymorphic bands. The genetic similarity matrix constructed with Jaccard’s coefficient using RAPD marker scores showed that the highest value was between H. psittacorum ‘Choconiana’ and H. psittacorum ‘Lady Di’ (0.384), while the lowest was between H. psittacorum ‘Choconiana’ and H. stricta ‘Dwarf Jamaican Red’ (0.244). Ten species and cultivars of Heliconia formed three distinct clusters at similarity coefficient value of 0.33, implying a parallelism between genetic and morphologic or taxonomic variability of Heliconia genotypes. ‘Golden Torch’, ‘Choconiana’, ‘Lady Di’ and H. wagneriana included in cluster I while ‘Choconiana’, ‘Lady Di’ formed a more cohesive entity. On the contrary, H. stricta var. ‘Dwarf Jamaican Red’ produced a separate cluster validating that taxonomically related entries clustered together and distant ones segregated. So, the current sequels demonstrate that RAPD is a rapid, relatively economical and functional for the characterization of genetic divergence between different Heliconia genotypes.
Malakar, M., Acharyya, P. and Biswas, S. (2020). Molecular characterization of ten Heliconia (Heliconiaceae) genotypes by means of RAPD markers. Acta Hortic. 1288, 95-102
DOI: 10.17660/ActaHortic.2020.1288.14
https://doi.org/10.17660/ActaHortic.2020.1288.14
Heliconia, DNA, molecular characterization, RAPD, cluster
English

Acta Horticulturae