In vitro mutagenesis efficiency with EMS (ethyl methanesulfonate) on Eustoma grandiflorum
The flower market in Mexico, in a growing boom and gradually gaining importance in the national economy, not only needs variety but quality, something that the floral market has left aside in Mexico to be mostly national consumption. Inside new possibilities, lisianthus (Eustoma grandiflorum) are flowers of interest in the international market due to its attractiveness for the consumers, slightly cultivated and original of warm areas of the southern region of the United States and the North of Mexico. It possesses attractive flowers and showy colors such as purple and yellowish, product of a wide range of flavonoids and anthocyanins. In order to obtain new forms and colors, mutagenesis has been often performed using physical and chemical ways, such as: gamma rays, X-rays or ethyl methanesulfonate (EMS), respectively. In this research we tested four concentrations of EMS (0.25, 0.5, 0.75 and 1 M) as inductor of genetic variability in four genotypes of lisianthus (C40, C43, C74, C83) in vitro. Also, we evaluated two times of application (1 and 2 h). Our results showed that EMS becomes toxic at 1 h and the survival percentage of plants decreased at 1 M. Likewise the survival percentage decrease in some plants was inversely proportional to increase in the concentration of EMS. The median lethal concentration was 0.5 M, in which a decrease was observed in size as well as rachitic and disordered growth. This demonstrates the high degree of toxicity of EMS and its capacity to induce changes, although these can be unspecific.
Mendoza-Gómez, R.J., Tapia-Campos, E. and Barba-Gonzalez, R. (2020). In vitro mutagenesis efficiency with EMS (ethyl methanesulfonate) on Eustoma grandiflorum. Acta Hortic. 1288, 163-166
anthocyanins, flavonoids, in vitro, lisianthus