Determination of genetic diversity among the fig (Ficus carica L.) genotypes using AFLP and SSR markers

Y. Yılmaz, H. İkten
Turkey is located within the origin of fig and very rich in terms of fig genetic resources. Plant genetic resources are useful for genetic diversity and evolutionary studies and breeding programs. In this study, genetic diversity among 64 fig genotypes, 32 males and 32 females were determined using AFLP and SSR markers. The 5' tail of EcoRI selective primers and reverse SSR primers were fluorescently labelled with IRDye-700 and IRD 800 fluorescent dyes. Following the denaturation, the PCR products were separated in polyacrylamide gel and visualized on a Li-Cor -IR2 4200 Genetic Analyzer (Li-Cor Biosciences). AFLP and SSR polymorphism were visually evaluated and scored in a binary way for band presence (1) or absence (0). There were total 142 alleles across the 42 loci with an average of 3.38 alleles per locus for SSR primers. Ten AFLP primer pair generated 905 fragments for 64 fig genotypes and 394 of which polymorphic, with minimum and maximum numbers of polymorphic bands obtained for each primer being 20 (EcoCCC-MseGAA) and 95 (EcoAGG-MseCAA), respectively. Similarity matrix and dendrogram were generated for each marker system separately and with combined data as well. The cluster analysis for both marker systems separated all genotypes successfully. The fig genotypes '530 İpek' - '288 İpek' were found to be closely related in both marker systems. A mantel test was performed by using the similarity matrices of two marker systems and an acceptable correlation (r=0.58) was obtained between the AFLP and SSR matrices.
Yılmaz, Y. and İkten, H. (2020). Determination of genetic diversity among the fig (Ficus carica L.) genotypes using AFLP and SSR markers. Acta Hortic. 1289, 281-290
DOI: 10.17660/ActaHortic.2020.1289.40
fig, phylogenetic relationship, molecular characterization, microsatellite markers

Acta Horticulturae