Progress in genetic, genomic and transcriptomic bases of fruit quality traits in apricot
One of the main objectives in the apricot breeding is the high fruit quality demanded by producers and consumers. Nowadays, the integration of marker-assisted selection in the breeding programmes is a powerful tool for the improvement of fruit quality traits and therefore is particularly useful in the development of new apricot varieties. In order to elucidate the molecular bases and to identify candidate genes associated with fruit quality traits and ripening process in apricot, a genetic, genomic and transcriptomic integrated approach is being developed at CEBAS-CSIC apricot breeding programme. In a first stage, two segregating progenies were phenotyped and genotyped by using molecular markers, allowing the construction of genetic linkage maps and the identification of quantitative trait loci associated to the most important quality traits. In a second stage, the transcriptomes of two contrasted genotypes from the same segregating progeny were sequenced at three developmental stages during fruit ripening. These RNA sequencing results allowed the identification of candidate genes involved in the main differential expressed pathways. In addition, monitoring the expression of those genes in reference apricot varieties allowed the establishment of a correlation between expression patterns and its specific phenotypes. With this integrated genomic and transcriptomic approach we have identified the candidate genes responsible for white/orange flesh color, anthocyanin content and carbohydrates metabolism.
Ruiz, D., García-Gómez, B.E., Salazar, J.A., Rubio, M. and Martínez-Gómez, P. (2020). Progress in genetic, genomic and transcriptomic bases of fruit quality traits in apricot. Acta Hortic. 1290, 63-68
apricot, QTL, RNA-Seq, RT-qPCR, quality traits, fruit ripening, candidate gene