Comparative transcriptome analysis reveals short-time heat stress negatively affects photosynthesis in Rosa chinensis 'Slater's Crimson China'

Z.Q. Li, C.X. Liu, S.Y. Shen, X.L. Jin, M.H. Zhang, Y.F. Wen, W. Xing
Previous studies have revealed a significant genetic relationship between high temperature and photosynthesis-related traits in rose. In this study, we used transcriptome profiling in combination with expression analysis and biochemical investigations to identify the underlying physiological and molecular responses in heat stress of Rosa chinensis 'Slater's Crimson China'. The expression analysis showed that most of the photosynthesis-related genes and DEGs related to the chlorophyll biosynthetic process were downregulated after 6 h of heat treatment. The results of chlorophyll fluorescence measurement indicated that photochemical quenching (qP) and non-photochemical quenching (NPQ) were relatively sensitive to high temperature, especially at early stage of heat stress. However, we also found several genes encoding crucial factors such as PGR5, PsbY and PsbP which are involved in protection of photosynthetic electron transport system, as well as CP12 and SCA1 gene coding proteins involved in protection of photosynthetic carbon fixation, were upregulated under heat stress treatment in R. chinensis. In summary, our findings indicated the regulation of adenosine triphosphate (ATP) synthesis, absorption and transportation of CO2, photosynthetic electron transport and levels of enzymes related to the Calvin cycle might be involved in the heat response mechanisms of R. chinensis.
Li, Z.Q., Liu, C.X., Shen, S.Y., Jin, X.L., Zhang, M.H., Wen, Y.F. and Xing, W. (2020). Comparative transcriptome analysis reveals short-time heat stress negatively affects photosynthesis in Rosa chinensis 'Slater's Crimson China'. Acta Hortic. 1291, 83-96
DOI: 10.17660/ActaHortic.2020.1291.11
https://doi.org/10.17660/ActaHortic.2020.1291.11
photosynthesis, comparative transcriptome analysis, chlorophyll fluorescence parameters, heat stress, R. chinensis
English

Acta Horticulturae