A novel dominant resistance gene for ToLCNDV in Cucurbita spp.

J. Romero, E. Aguado, C. Martínez, A. García, C. Cebrián, H.S. Paris, M. Jamilena
Tomato leaf curl New Delhi virus (ToLCNDV), is a whitefly transmitted bipartite Begomovirus (family Geminiviridae) causing a great damage of Zucchini squash in SE Spain. The virus produces very characteristic symptoms, including yellowing and curling of leaves, and inhibition of plant growth and development. The identification of new sources of resistance is one of the challenges for disease control. To achieve this goal, we performed a screening of a collection comprising 143 Cucurbita accessions with a stable population of viruliferous whiteflies. The seven most tolerant accessions in the field were inoculated mechanically, and one of them (BSUAL-252) was confirmed to be highly resistant to the virus, showing absence of symptoms at 20 and 30 days after inoculation, and a low accumulation of the virus DNA as detected by qPCR in plant apical shoots. The segregation of susceptible and resistant plants in F1 and F2 generations demonstrated that the resistance is conferred by a single dominant gene. This dominant ToLCNDV resistance in BSUAL-252 makes this accession suitable for future breeding programs in Cucurbita crop species, including zucchini squash (Cucurbita pepo).
Romero, J., Aguado, E., Martínez, C., García, A., Cebrián, C., Paris, H.S. and Jamilena, M. (2020). A novel dominant resistance gene for ToLCNDV in Cucurbita spp.. Acta Hortic. 1294, 233-238
DOI: 10.17660/ActaHortic.2020.1294.29
C. moschata, C. pepo, ToLCNDV resistance, dominant gene

Acta Horticulturae