Genetic divergence in pumpkin genotypes using ISSR markers

R.M.E. Borges, N.F. de Melo
The objective of this work was to estimate the genetic divergence in 12 pumpkin genotypes as of 2016, using 17 inter simple sequence repeats molecular markers. These were efficient in detecting polymorphism in the evaluated genotypes, amplifying a total of 2671 bands. The number of polymorphic bands per initiator varied from one in primers TriTGT3'YC and TriCCG3'RC to 77 in initiator TriGAC3'RC. Divergence analysis by UPGMA clustering of the data allowed the definition of four contrasting groups with similarities of 49.73, 45.74, 36.24 and 31.72%, respectively; confirming that genetic variability among the evaluated genotypes can be explored, as well as advances in pumpkin improvement can be possible. The most divergent genotypes were C. moschata 6 and C. moschata 4, with divergences of 77.6 and 100%, respectively.
Borges, R.M.E. and de Melo, N.F. (2020). Genetic divergence in pumpkin genotypes using ISSR markers. Acta Hortic. 1294, 29-34
DOI: 10.17660/ActaHortic.2020.1294.5
Cucurbita moschata, Cucurbitaceae improvement, variability, molecular marker, Brazilian Northeast

Acta Horticulturae