Effects of BA, NAA, and activated charcoal on micropropagation of Nepenthes mirabilis (Lour.) Druce

Nepenthes mirabilis (Lour.) Druce, a carnivorous plant in the Nepenthaceae family, has been brought out of nature for a large amount for commercial purposes. This study aims to investigate the influence of plant growth regulators (BA, NAA) and activated charcoal on micropropagation of Nepenthes mirabilis for conservation. The sterile young shoots were cultured on Murashige and Skoog (MS) medium containing BA supplemented with NAA and activated charcoal at different concentrations for 4 weeks. The results showed that the MS medium supplemented with 2 mg L^‑1 BA, 0.2 mg L^‑1 NAA, and 0.25% activated charcoal is the suitable condition for shoot induction, 8.48±1.12 shoots explant^‑1. The MS medium with 1 mg L^‑1 BA, 0.2 mg L^‑1 NAA, and 0.25% activated charcoal gave the highest number of leaf, 10.45±1.05 leaves explant^‑1. The MS medium supplemented with 1 mg L^‑1 BA, 0.1 mg L^‑1 NAA, and 0.5% activated charcoal gave the highest plant height (2.72±0.26 cm) and pitcher production(1.60±0.51 pitchers explant^‑1).