In vitro culture of Enhalus acoroides (L.F.) Royle
In vitro cultures of E. acoroides, a very important tropical seagrass in terms of biomass production, were assessed for marine conservation and commercial production for restoration projects. Leaves, rhizomes, and seeds of E. acoroides collected from Para Bay, Phuket, Thailand were surface sterilized and maintained on artificial sea water under sterile conditions. After 7 days of culture, only seeds got 0% contamination; while, leaf and rhizome preparations got 100 and 50% contamination, respectively. The fruits were separated into 4 stages ranked by their sizes. The fruit at stages 2 and 3 (25-35 and 15-25 mm in diameter, respectively) provided higher germination and growth. They were collected and cultured on the artificial sea water and natural sea water media containing 3% sucrose. The results showed that even though there was no effect of sucrose on E. acoroides seed germination, it affected root hair production. The suitable conditions for multiple shoot induction needs to be further studied.
Meksuwan, Y., Sutthinon, P. and Ritchie, R.J. (2020). In vitro culture of Enhalus acoroides (L.F.) Royle. Acta Hortic. 1298, 287-292
E. acoroides, seed germination, seagrass tissue culture, synthetic sea water