Competent callus induction from shoot segments of Sangmon 'Nuan Rajinee' bamboo (Dendrocalamus sericeus Munro)
Callus was induced from 1 cm long of Sangmon 'Nuan Rajinee' bamboo; subsequently shoot multiplication was produced from callus using MS medium supplemented with or without 2,4-D, BA, NAA, and kinetin. Our results indicated that there was no callus formation on MS medium without plant growth regulators. However, when BA at 1.0 mg L‑1 or kinetin at 2.0 mg L‑1 in combination with 2,4-D at 3.0 mg L‑1 were added and supplemented with or without casein hydrolysate, L-proline, and L-glutamine at a concentration of 500 mg L‑1, 73.3-86.6% callus was found in all media. Afterwards, callus was transferred to shoot induction medium. The results showed that MS media supplemented with 1.0 mg L‑1 BA and 0.1 mg L‑1 TDZ gave the highest number of shoots at 12.4 shoots, significantly different from number of shoots obtained from other treatments.
Karawak, P., Obsuwan, K., Sengsai, S. and Thepsithar, C. (2020). Competent callus induction from shoot segments of Sangmon 'Nuan Rajinee' bamboo (Dendrocalamus sericeus Munro). Acta Hortic. 1298, 293-298
micropropagation, multiple shoots, auxin, cytokinin, proline, glutamine, casein hydrolysate