Competent callus induction from shoot segments of Sangmon 'Nuan Rajinee' bamboo (Dendrocalamus sericeus Munro)

Callus was induced from 1 cm long of Sangmon 'Nuan Rajinee' bamboo; subsequently shoot multiplication was produced from callus using MS medium supplemented with or without 2,4-D, BA, NAA, and kinetin. Our results indicated that there was no callus formation on MS medium without plant growth regulators. However, when BA at 1.0 mg L^‑1 or kinetin at 2.0 mg L^‑1 in combination with 2,4-D at 3.0 mg L^‑1 were added and supplemented with or without casein hydrolysate, L-proline, and L-glutamine at a concentration of 500 mg L^‑1, 73.3-86.6% callus was found in all media. Afterwards, callus was transferred to shoot induction medium. The results showed that MS media supplemented with 1.0 mg L^‑1 BA and 0.1 mg L^‑1 TDZ gave the highest number of shoots at 12.4 shoots, significantly different from number of shoots obtained from other treatments.