Effects of some organic additives on shoot initiation and shoot multiplication of Sangmon 'Nuan Rajinee' bamboo (Dendrocalamus sericeus Munro)

Shoot initiation from nodal segments and shoot multiplication from a shoot cluster of Sangmon 'Nuan Rajinee' bamboo (Dendrocalamus sericeus Munro) using some organic additives supplemented in culture media was carried out. A surface-sterilized nodal segment was cultured for 3 weeks on modified solid Murashige and Skoog (MS) medium, containing 3.0 mg L^‑1 benzyladenine (BA) and 0.1 mg L^‑1 thidiazuron (TDZ), added with 2.0 g L^‑1 tryptone or 0.5 mg L^‑1 biotin or 0.1 mg L^‑1 folic acid or 1.0 g L^‑1 monosodium glutamate (MSG). MSG in the solid medium gave better result in number of shoots (3.0 shoots node^‑1) with greener and healthier shoots than those from other treatments. For shoot multiplication, a shoot cluster, as explant, was cultured on modified liquid MS medium (containing 1.0 mg L^‑1 BA, 0.1 mg L^‑1 TDZ and 0.5 g L^‑1 L-proline) supplemented with different concentrations of MSG (0.5, 1.0, 2.0 and 3.0 g L^‑1) or L-glutamine (0.1, 0.2, 0.3 and 0.4 g L^‑1) compared to 0.04 mg L^‑1 adenine sulfate as a positive control. It was found that medium supplemented with 1.0 mg L^‑1 MSG gave the highest multiplication rate of 3.7-folds from shoots of initial explant. Furthermore, medium supplemented with 0.1 and 0.2 g L^‑1 L-glutamine also gave the high multiplication rate of 3.4-folds from shoots of initial explant. The same results of medium added with 0.1 g L^‑1 L-glutamine were found on medium containing 0.04 g L^‑1 adenine sulfate with a cluster of small green shoots.