Effect of plant growth regulators and agar concentration on shoot multiplication and hyperhydricity of Anthyllis barba-jovis
The increase of agar concentration and the addition of auxin into the shoot proliferation medium were tested for their effectiveness in eliminating hyperhydricity in Anthyllis barba-jovis L. during micropropagation. Single node explants of adult- or seedling-origin were cultured on MS medium supplemented with 4.0 mg L‑1 6-benzyladenine (BA) in combination with 0.0, 0.1 or 0.5 mg L‑1 1-naphthaleneacetic acid (NAA) solidified with 8.0 or 12.0 g L‑1 agar. Hormone free-MS medium with 8.0 g L‑1 agar, used as control, produced the lowest shoot number and length (1.0 shoot explant‑1, 0.6-0.8 cm), and no hyperhydricity. Both explant types responded in a similar way in all treatments. MS medium with BA solidified with 8 g L‑1 agar induced the highest shoot proliferation (12.5 shoots explant‑1, 1.8 cm shoot length) but more than 75% of the shoots were hyperhydric. The addition of NAA (independently of concentration) into the BA-medium, or the increase of agar concentration resulted in reduced hyperhydricity, and the combination of both almost eliminated it. In BA-NAA-media, the highest number of normal shoots was produced when BA was combined with 0.1 mg L‑1 NAA and 12.0 g L‑1 agar (7.6 or 7.9 shoots explant‑1 of adult and seedling origin, respectively). Both agar increased and NAA reduced slightly shoot length (1.0-1.2 cm). Therefore, increasing agar concentration to 12.0 g L‑1 and supplementing the medium with 4.0 mg L‑1 BA and 0.1 or 0.5 mg L‑1 NAA, resulted in the elimination of hyperhydricity and in the best shoot multiplication response.
Vlachou, G., Trigka, M. and Papafotiou, M. (2020). Effect of plant growth regulators and agar concentration on shoot multiplication and hyperhydricity of Anthyllis barba-jovis. Acta Hortic. 1298, 341-346
micropropagation, explant origin, 6-benzyladenine (BA), 1-naphthaleneacetic acid (NAA), hyperhydricity, agar concentration, native plants