Effects of BA and TDZ for in vitro shoot multiplication of three Hedychium species
Hedychium is a terrestrial zingiberaceous species with underground rhizomes, as a source of raw materials for pharmaceutical and cosmetic products. Therefore, demand for raw plant material has increased, resulting in overcollection from the wild. In this study, two plant growth regulators (PGRs) were used for in vitro shoot multification of three Hedychium species. The sterilized young shoot tips of H. stenopetalum, H. neocarneum, and H. flavescens were cultured on Murashige and Skoog (MS, 1962) supplemented with various concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ). All cultures were incubated under 16 h illiumination day‑1 at 25±2°C for 8 weeks. The results showed that the medium supplemented with 2.7 µM TDZ gave the highest shoot multiplication of H. flavescens and H. stenopetalum at 5.7 and 5.9 shoots explant‑1, respectively. The medium supplemented with 0.9 µM TDZ gave the highest multiplication of 5 shoots explant‑1 in H. neocarneum. This significant study could be applied for future large-scale production.
Klaharn, P., Pumisutapon, P., Songnun, K. and Rodpradit, S. (2020). Effects of BA and TDZ for in vitro shoot multiplication of three Hedychium species. Acta Hortic. 1298, 353-358
Zingiberaceae, micropropagation, plant growth regulator, cytokinin