Effect of different combinations of NAA and TDZ for shoot induction in vitro culture of Aglaonema simplex (Blume) Blume

Wan Khanmak (Aglaonema simplex (Blume) Blume) is a kind of Thai herb. The ripe fruits are extensively used as longevity enhancer and anti-asthma medication, but the number of fruits plant^‑1 was only 3-14. This research project aims to find the most appropriate tissue culture protocol for mass propagation of Wan Khanmak herb. The experimental design was completely randomized design (CRD). The axillary buds of Wan Khanmak were incubated on MS medium supplemented with 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) at various doses; 0:0, 0.25:0.5, 0.25:1.0, 0.25:1.5, 0.25:2.0 and 0.25:2.5 mg L^‑1. The results showed that the most effective medium was MS medium supplemented with NAA and TDZ at 0.25:2.5 mg L^‑1 ratio which produced the highest number of average shoots per culture (4.49 shoots) with an average shoots length 1.60 cm in 8 weeks of culture. This treatment gave the fastest time to produce the first shoot in 8.5 days.