Evaluation of phenotyping and genotyping for resistance against Tomato yellow leaf curl Thailand virus (TYLCTHV) validated by Ty-2, Ty-3, and Ty-4 genes in tomato
Tomato yellow leaf curl virus (TYLCV) caused by whitefly-transmitted begomoviruses is one of the most destructive and causing heavy losses in tomato yields, especially in sub-tropic and tropic regions including Thailand. Therefore, breeding a tomato cultivar resistant to TYLCV is necessary. The purpose of this research was to evaluate tomato cultivars which are resistant to TYLCV for Thailand's strain (TYLC-THV) with agronomic traits. The 35 tomato accessions obtained from The World Vegetable Center (AVRDC) were compared with the susceptible cultivar (Seedatip: KKU-T12002). The results of field evaluation showed that the 11 tomato accessions performed moderately resistant to TYLC-THV and their good agronomic traits. The results for resistance were classified into 3 groups based on their responses to TYLC-THV. The first group, i.e., KKU-T24003 and KKU-T23160 were identified as highly resistant. The second group, i.e., KKU-T23176, was identified as resistant and the other seven tomato cultivars were identified as moderately resistant. In addition, Ty-2, Ty-3, and Ty-4 gene were validated by 2 SCAR markers and 1 SSR marker in the 12 tomato accessions.
Krupeu, K., Techawongstien, S. and Lapjit, C. (2020). Evaluation of phenotyping and genotyping for resistance against Tomato yellow leaf curl Thailand virus (TYLCTHV) validated by Ty-2, Ty-3, and Ty-4 genes in tomato. Acta Hortic. 1298, 511-516
whitefly, SCAR markers, SSR marker