The effects of seed coat scarification, priming, and cold stratification on seed germination of a tropical night-blooming waterlily hybrid Nymphaea rubra 'Maeploi'
Nymphaea rubra 'Maeploi' is a naturally tropical waterlily hybrid. It was discovered by Dr. Slearmlarp Wasuwat and then registered to the International Waterlily and Water Gardening Society (IWGS) in 2002. This waterlily hybrid cultivar has more attractive characteristics, such as the bright to shocking pink petals and reddish-brown leaves. In this research, we aimed to study the effects of seed coat scarification, seed priming, and cold stratification on germination of N. rubra 'Maeploi' seeds. Clipped seeds cultured under light condition gave better germination (41.7%) than the other conditions. Seeds were investigated by hydropriming (distilled water and filtered water) and osmopriming (sucrose, glucose, maltose, and sorbitol) treatments at different concentrations (0, 1, 2, 4, 8, 16, and 32% (w/v)). After 4 weeks of culture, the germination of primed seeds cultured in filtered water was 68.3% out of a total of 90% viable seeds. Non-clipped seeds cultured for four weeks without cold stratification (i.e., at 25°C) gave better germination (78.3%) than the seeds cultured with cold stratification (10%). The identified preconditioning treatments provide basic information and a starting point for future research on the ex situ conservation, propagation, and breeding experiment of tropical night-blooming waterlilies and their hybrids.
Khaenthong, I., Phairin, U., Mattisinlapin, P., Subudom, S., Sripan, S., Boonthongto, A. and Malaithong, S. (2020). The effects of seed coat scarification, priming, and cold stratification on seed germination of a tropical night-blooming waterlily hybrid Nymphaea rubra 'Maeploi'. Acta Hortic. 1298, 581-586
germination, scarification, priming, stratification, Nymphaea rubra 'Maeploi'