PCR-SSCP analysis and disease control by the application of biological and chemical agents in asparagus decline
In asparagus (Asparagus officinalis L.) producing regions in Japan, F. oxysporum f. sp. asparagi and F. proliferatum are some of the most important causes as biotic factors which lead to asparagus decline. In this study, the diversity of Fusarium population which cause Fusarium crown and root rot in asparagus decline field was estimated by PCR-SSCP (single-stranded conformational polymorphism) method. In addition, the effects of combination use of biological (arbuscular mycorrhizal fungi (AMF) and non-pathogenic Fusarium oxysporum (NPFO)) and chemical (NaCl 50, 100 mM) control agents on disease and growth in the decline field was investigated. As for Fusarium diversity in the decline field, population of F. oxysporum f. sp. asparagi and F. proliferatum are 55.6 and 44.4%. Rate of withered plants with root rot and growth reduction was 60% in control plots 12 weeks after transplanting. However, single and combination use of AMF, NPFO and NaCl plots had a much lower rate of withered plants with root rot and growth reduction, and AMF + NaCl 100 mM plots showed the lowest rate in yellowing of ferns among all the plots. Highest plant height and number of ferns was observed in AMF + NaCl plots. These results suggest that PCR-SSCP can rapidly and objectively identify Fusarium associated with asparagus decline, and the combination use of AMF, NaCl and NPFO is effective for control of disease and growth in decline field.
Liu, J., Taniguchi, A., Matsubara, Y., Kojima, T. and Takahashi, K. (2020). PCR-SSCP analysis and disease control by the application of biological and chemical agents in asparagus decline. Acta Hortic. 1301, 149-154
Fusarium population, growth promotion, arbuscular mycorrhizal fungi, non-pathogenic Fusarium oxysporum, NaCl