Identification and expression analyses of MAP kinase gene PaMAK1 in Phomopsis asparagi

Y. Zhang, G. Chen, H. Qu, J. Zhou, S. Luo, Y. Tang, S. Zhang
Asparagus stem blight is a highly destructive disease of asparagus worldwide, especially in China and some Asian countries. Filamentous fungus Phomopsis asparagi is identified as the causing pathogen. However, its molecular pathogenic mechanism is poorly known. Identification of pathogenic related genes is of great importance for understanding of the molecular pathogenic mechanism on asparagus stem blight. It could be considered as potential molecular targets for asparagus stem blight control and the results will also provide a solid gene foundation and new reference strategy for disease control and disease resistance breeding. MAPK (mitogen-activated protein kinase) is a class of eukaryotic serine/threonine protease closely related with extracellular signal transduction, cell growth, development, and differentiation. MAPK signaling pathway is an important way for study of pathogenic fungi pathogenic mechanism. A new FUS3/KSS1 MAPK gene was identified from asparagus stem blight fungus pathogen P. asparagi and named PaMAK1. Its full genomic DNA was 1309 bp in length with four exons and three introns. It contained an open reading frame (ORF) of 1068 bp and encoded a protein of 355 amino acids. The Blast analysis showed its amino acid sequence had high homology to FvMK1, GPMK1, and PMK1. The result of qRT-PCR showed that PaMAK1 was all transcribed during hyphal growth, conidia, and pycnidia. In the infection process, the expression level of PaMAK1 was about 2.28-fold in comparison with that of the hyphal stage. The results provided a foundation to illustrate function of FUS3/KSS1 class MAPK homologous gene in asparagus stem blight fungus.
Zhang, Y., Chen, G., Qu, H., Zhou, J., Luo, S., Tang, Y. and Zhang, S. (2020). Identification and expression analyses of MAP kinase gene PaMAK1 in Phomopsis asparagi. Acta Hortic. 1301, 155-162
DOI: 10.17660/ActaHortic.2020.1301.24
Phomopsis asparagi, PaMAK1, identification, gene expression

Acta Horticulturae