Genetic diversity of 24 asparagus (Asparagus officinalis) accessions as assessed by ISSR markers
Asparagus officinalis L. is a dioecious perennial plant globally known for its fine flavor and high nutritional value. In this study, inter-simple sequence repeat (ISSR) marker analysis was used to evaluate genetic diversity in 24 asparagus accessions of world-wide origin. Results showed that 74 (75.61%) of the 98 DNA fragments amplified by 10 primers using ISSR-PCR were polymorphic. Each primer amplified averagely 10 DNA fragments. Genetic similarity coefficient were determined, and a dendrogram was then constructed using the unweighted pair-group method. Accessions from China, Italy, and USA were distinguished, but the Netherlands and USA accessions grouped together. The results from the principal component and similarity coefficient analyses were relatively consistent. Thus, the ISSR markers employed were useful in distinguishing A. officinalis diversity confirming the importance of future genetic studies.
Chen, Helong, Dai, Zhenzhen, Gao, Jianming, Zhang, Shiqing, Zheng, Jinlong, Xi, Jingen and Yi, Kexian (2020). Genetic diversity of 24 asparagus (Asparagus officinalis) accessions as assessed by ISSR markers. Acta Hortic. 1301, 31-36
king of vegetables, inter-simple sequence repeat, polymorphic, diversity analysis, principal coordinate analysis, clustering