Fig viruses in Bosnia and Herzegovina
Field surveys were carried out in the Herzegovina region during the spring of 2015 and 2016, in fig germplasm collections, fig orchards and outdoor gardens in Mostar, Trebinje, Ljubuki and Grude. A total of 49 fig leaf samples were collected from plants showing different symptoms associated with fig mosaic disease and asymptomatic plants. Total nucleic acids were extracted from leaf midribs and tested by RT-PCR for the presence of FMV, FLMaV-1, FLMaV-2, FMMaV, FLV-1, FCrV, and FFkaV, while the presence of FBV-1 was assessed with PCR using specific primers. The analyses revealed the presence of FMV, FLMaV-1, FLMaV-2, FMMaV, FFkaV and FBV-1 in collected fig samples. The most common infections were with FMV, FLMaV-1 and FBV-1. Moreover, mixed infections involving two or three viruses were frequently found. Sequencing analyses results showed that Herzegovina isolates shared 83-99% nt identity with other isolates of the same viruses stored in GenBank. The virus status of this crop is probably compromised and a sanitation programme is required to produce healthy plant material.
Delić, D., Elbeaino, T., Lolić, B. and Đurić, G. (2021). Fig viruses in Bosnia and Herzegovina. Acta Hortic. 1308, 319-324
Ficus carica, virus diseases, RT PCR, PCR, Herzegovina