Investigation of S-incompatibility breakdown in 'Karina' sweet cherry
The paper presents results of a two-year study of self-(in)compatibility in Karina, previously reported as a self-incompatible sweet cherry cultivar. After emasculation and bagging, pistils of Karina were selfed and pollinated with the nominally cross-incompatible cultivars Germersdorfer 1 and Lambert at the beginning of full flowering. In the same way, the pollen of Karina was used to pollinate Germersdorfer 1 and Lambert. PCR analysis to determine S-genotypes of Karina, Germersdorfer 1 and Lambert using consensus and allele-specific primers for S-RNase resulted in patterns consistent with S3S4 genotype. After selfing, Karina behaved as self-compatible, since its pollen tubes reached the base of the style and ovary, penetrating the nucellus and fruit set was recorded in both years. Pollen tubes penetrating the nucellus and fruit set were also recorded after pollination with Germersdorfer 1 and Lambert. In contrast, Germersdorfer 1 and Lambert did not set any fruit when pollinated with Karina. Microscopic observation showed pollen tubes ending the growth in the upper and middle third of the style. The behaviour of Karina after selfing, as well as its behaviour as the pollen donor/recipient in pollination with cultivars of the same S-allele constitution requires further molecular and genetic analysis.
Radičević, S., Marić, S., Cerović, R. and Đorđević, M. (2021). Investigation of S-incompatibility breakdown in 'Karina' sweet cherry. Acta Hortic. 1308, 53-60
Prunus avium L., S-genotype, pollination, incompatible pollen tubes, fruit set