Investigating the composition and the anti-proliferative mechanism of a strawberry (Fragaria × ananassa) methanolic extract on in vitro murine breast cancer cells

L. Mazzoni, T.Y. Forbes-Hernandez, F. Capocasa
The aim of this study was the evaluation of the effects of strawberry extracts treatment on two in vitro models of murine breast cancer cell line, trying to detect a specific pathway (AMPK) through which strawberries exert their anticancer activity. Firstly, a characterization of the sensorial and nutritional quality of 'Alba' strawberry cultivar was performed. Then, the anticancer activity of methanolic extract from 'Alba' on two murine cancer cell lines, N202/1A (with high levels of HER2/neu oncogene) and N202/1E (with low levels of HER2/neu oncogene) was evaluated after 48 h of treatment through the expression of several proteins related to apoptosis, autophagy, metastasis, oxidative status, mitochondrial functionality, oncogene expression and AMPK pathway. This study demonstrated that a well characterized strawberry extract possessed an antiproliferative effect on cancer cells, through the induction of apoptosis and oxidative stress. At the same time, a relation between the AMPK pathway and the anticancer effects of strawberries was demonstrated.
Mazzoni, L., Forbes-Hernandez, T.Y. and Capocasa, F. (2021). Investigating the composition and the anti-proliferative mechanism of a strawberry (Fragaria × ananassa) methanolic extract on in vitro murine breast cancer cells. Acta Hortic. 1309, 1009-1016
DOI: 10.17660/ActaHortic.2021.1309.143
https://doi.org/10.17660/ActaHortic.2021.1309.143
strawberry, antioxidants, polyphenols, anthocyanins, gene expression, western blot
English

Acta Horticulturae