Use of RAPD and SCAR markers for identification of strawberry genotypes with red stele resistance genes Rpf1 and fruit rot resistance genes Rca2 in the hybrid progenies

The strawberry diseases such as anthracnose fruit rot caused by Colletotrichum acutatum Simmonds and red stele root rot caused by Phytophthora fragariae Hickman are spreading more and more in Europe in all areas where strawberries are grown. Molecular identification of different clones is an important tool for effective and useful management of strawberry germplasms. This study reports on the use of RAPD and SCAR markers for identification of different genotypes with red stele root rot resistance allele Rpf1 and anthracnose fruit rot resistance allele, Rca2, in 42 progenies from the cross of 'Benton' × 'Cambridge Favourite'. The results showed that: i) the molecular marker STS-Rca2_240, associated with resistance allele Rca2, was detected in 17 descendants, representing 40.5%; ii) both resistance alleles Rpf1 and Rca2 were detected in 9 genotypes from 42 descendants (21.43%).