Genome-wide prediction of powdery mildew resistance in the octoploid strawberry

R. Tapia, L.F. Osorio, S. Verma, S. Lee, V.M. Whitaker
Powdery mildew (PM) caused by Podosphaera aphanis is a major fungal disease in cultivated strawberry. Characterization of resistance loci has been challenging for breeders and geneticists due to the complex and polygenic nature of resistance. Genome-wide prediction (GWP) utilizes genome-wide markers to predict the performance of individuals and accelerate genetic gains for polygenic traits. In the present study, we implemented GWP models using six pedigree-connected full-sib families from crosses among four University of Florida advanced selections. These families were evaluated in a replicated field trial, and 250 to 9,000 polymorphic single nucleotide polymorphism (SNP) markers were chosen based on genome coverage and polymorphism. Half-sibs were used as a training population to rank seedlings within each cross (full-sib family). The highest predictive abilities using Bayes B, GBLUP and RKHS methods were 0.36, 0.38 and 0.38, respectively. The three methods had comparable parent selection efficiencies, and within-family genetic gains were at least 10% over the family mean when selecting the top 10% of seedlings ranked by GWP. As few as 500 SNP markers were sufficient to achieve comparable results. Overall, this study demonstrates the potential for application of within-family GWP for rapid genetic improvement of powdery mildew resistance in strawberry seedling families.
Tapia, R., Osorio, L.F., Verma, S., Lee, S. and Whitaker, V.M. (2021). Genome-wide prediction of powdery mildew resistance in the octoploid strawberry. Acta Hortic. 1309, 101-106
DOI: 10.17660/ActaHortic.2021.1309.16
powdery mildew, polygenic inheritance, genome-wide selection, strawberry

Acta Horticulturae