Isolation and analysis of flavonoid 3'-hydroxylase (F3'H) genes from cyclamen 'Strauss'
Anthocyanins are the most abundant and primary flavonoid pigments, which produce a wide range of colors. Flavonoid 3-hydroxylase (F3H) is one of the key enzymes in anthocyanin biosynthesis and has been identified from a variety of ornamental plants. For isolating and charactering the anthocyanin related F3H in cyclamen, three candidates of F3Hs named as STRF3H1, STRF3H2a and STRF3H2b were obtained from petals of Cyclamen persicum cultivar Strauss (STR). Phylogenetic analysis and sequence alignment suggested that these putative STRF3Hs belong to the CYB75B class of cytochrome P450 superfamily, containing the unique conserved motifs of F3H enzyme. The prediction of transmembrane structure showed that STRF3H2a and STRF3H2b each had a transmembrane region, while F3H1 did not. STR and the wild Cyclamen persicum (C. persicum), have different major anthocyanin components in petals. Since the synthesis of major anthocyanins in C. persicum and STR are closely related to F3H and flavonoid 3,5-hydroxylase (F35H), the expression of these genes had been compared by real-time PCR. F3Hs were expressed strongly in STR, weakly in C. persicum, while F35H was opposite. The results implied F3H is likely taking an active role in pigmentation in STR. The function will be further analyzed to verify the relevance between F3H and STR flower color formation.
Kang, X.F., Akita, Y. and Mikami, R. (2021). Isolation and analysis of flavonoid 3'-hydroxylase (F3'H) genes from cyclamen 'Strauss'. Acta Hortic. 1312, 105-110
anthocyanin, cloning, flower color, pigment