The hormonal control of callus induction in 'GM256' × 'M9' progeny of different stem rooting ability
The objective of this research was to find an efficient protocol to improve callus induction in apple rootstocks.
Concentrations and ratios of auxin and cytokinin were investigated on 'GM256' × 'M9' progenies having either low or high rooting ability.
Specifically, leaves of plants with different stem cutting rooting abilities were cultured for 21 days on Murashige and Skoog (MS) semisolid medium containing 2, 3 and 4 mg L‑1 thidiazuron (TDZ) in combination with 0.2, 0.35 and 0.5 mg L‑1 1-naphthaleneacetic acid (NAA) in the dark.
Regeneration ability was positively correlated with stem cutting rooting ability.
Easy-to-root progeny had a lower browning percentage and a higher callus formation percentage when compared with hard-to-root progeny.
NAA was the main factor affecting hard-to-root progeny, while TDZ had a significant effect on easy-to-root progeny.
Shoots were induced only in easy-to-root progeny.
In conclusion, callus formation of progenies with both high and low rooting ability was promoted when cultured with 0.5 mg L‑1 NAA + 2 mg L‑1 TDZ.
Zhang, Z., Zhang, L., Zhou, Y., Tian, X., Xu, X., Wang, Y., Wu, T., Zhang, X., Li, W., Qiu, C. and Han, Z. (2021). The hormonal control of callus induction in 'GM256' × 'M9' progeny of different stem rooting ability. Acta Hortic. 1312, 131-138
DOI: 10.17660/ActaHortic.2021.1312.20
https://doi.org/10.17660/ActaHortic.2021.1312.20
DOI: 10.17660/ActaHortic.2021.1312.20
https://doi.org/10.17660/ActaHortic.2021.1312.20
apple rootstocks, tissue culture, callus induction, rooting ability
English
1312_20
131-138
- Division Tropical and Subtropical Fruit and Nuts
- Division Plant Genetic Resources and Biotechnology
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Postharvest and Quality Assurance
- Division Horticulture for Human Health
- Division Horticulture for Development
- Division Precision Horticulture and Engineering
- Division Landscape and Urban Horticulture
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems