Transcriptome analysis of the effect of modified atmosphere packaging and low temperature on the mature green tomatoes during storage

T. Shiina, D. Ciptaningtyas, T. Vanitha, N. Benyakart, H. Umehara, M. Thammawong
Tomato is a major vegetable crop in the world. It is rich in potassium, vitamin A, vitamin B, vitamin C, lycopene and beta-carotene. Tomato is regularly consumed in the diet, having a defensive role for reducing the risk of some types of cancer and ischemic heart disease. It is reported that the huge amount of produce, including tomato, is discarded due to postharvest losses caused by quality deterioration. Low storage temperature and modified atmosphere packaging (MAP) have the potential to keep the quality of tomato after harvest. The objective of this investigation was to analyze the effect of low temperature (15°C) and MAP on the ripening and the transcription profile of 'KEK-1' tomatoes. Mature green tomatoes var. 'KEK-1' were stored at 25°C with MAP or without MAP (perforated film package) or stored at 15°C without MAP. The packaging materials used were low-density polyethylene (LDPE) film (40 µm). The O2 concentration of 3 to 5% in the MA packaged tomatoes was maintained throughout the storage period. Microarray analysis was conducted using total RNA extracted from tomato samples, before storage and from the three different storage conditions for five days. The pericarp color value of the tomatoes and the ripening related genes expression from four treatments confirmed a clear difference between the ripening stages among samples. Comparison of the gene expression profile was conducted by using microarray data analysis software. The effects of storage and storage conditions applied in this study were clearly seen by a profile plot of genes. A principal component analysis using three components showed the clear difference among the four samples. The gene showing a distinctive expression pattern among samples was selected and genes showing a similar pattern were also selected. Four distinctive expression patterns were found and genes that have similar pattern were grouped. There is no overlap among the groups, suggesting these groupings reflect different biological status. The results showed that several ripening related genes (including ACC synthase and ACC oxidase, histidine decarboxylase, alcohol acyl transferase) and defense reaction related genes (including chitinase, pathogenesis related leaf protein 6, PR-1a1 protein) were up/downregulated by low temperature or MA packaging.
Shiina, T., Ciptaningtyas, D., Vanitha, T., Benyakart, N., Umehara, H. and Thammawong, M. (2021). Transcriptome analysis of the effect of modified atmosphere packaging and low temperature on the mature green tomatoes during storage. Acta Hortic. 1312, 37-44
DOI: 10.17660/ActaHortic.2021.1312.6
tomato, ripening, MAP, low temperature, gene expression, microarray

Acta Horticulturae