Establishment of a micropropagation method for in vitro rooting of Callistemon viminalis micro-cuttings

S. Shokri, H. Zarei, M. Alizadeh
A micropropagation method was described for bottlebrush (Callistemon viminalis) using single node explants under in vitro conditions. This method provided a rapid, reliable, repeatable mass propagation method. Multiple shoots were induced from axillary buds on Murashige and Skoog (MS) and Anderson's Rhododendron Medium (ARM) media containing different concentrations of 6-Benzylaminopurine (BAP) in combination with naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA). Both MS and ARM media were suitable for these goals and the ARM medium with BAP (2 mg L‑1) and NAA (0.2 mg L‑1) was the most effective medium for shoot multiplication, while the ARM medium is better than the MS medium for shoot proliferation. In vitro regenerated shoots on half-strength, MS media supplemented with IBA (1.5 mg L‑1) developed better roots (100%), while no significant difference was evident between half-strength MS and MS medium containing NAA (0.2 mg L‑1). The rooted plantlets were successfully acclimatized and the most viability of rooted shoots (100%) was observed with MS media supplemented with IBA (1.5 mg L‑1) and half-strength MS medium supplemented with NAA (0.2 mg L‑1) also.
Shokri, S., Zarei, H. and Alizadeh, M. (2021). Establishment of a micropropagation method for in vitro rooting of Callistemon viminalis micro-cuttings. Acta Hortic. 1315, 257-264
DOI: 10.17660/ActaHortic.2021.1315.39
https://doi.org/10.17660/ActaHortic.2021.1315.39
multiplication, tissue culture, single node, in vitro, bottlebrush
English

Acta Horticulturae