Lettuce yield and nutritive value as affected by a biopolymer microparticles application
Lettuce (Lactuca sativa L.) is economically the most important leafy vegetable. Besides the high yield, the focus is on the production of lettuce with high nutritive value, rich in phytochemicals important for human health. One of the methods to improve agronomic and qualitative traits is the application of microparticles containing chemical and/or biological agents. A study was conducted to test the application of different microparticle formulations on soil-grown lettuce. Eight types of biopolymer microparticles made of sodium alginate as a carrier with or without chitosan coating, with active ingredients (Ca2+, Cu2+, Ca2+/Trichoderma viride, Cu2+/T. viride), T. viride spores suspended in 0.85% saline solution and untreated control were tested. A one-factorial trial with the Batavia lettuce cultivar 'Melina' was set in a completely randomized block design in three repetitions. Four grams of microparticles were incorporated into the soil together with seedling planting. Lettuce biomass, marketable rosette weight, and rendement which represents the marketable rosette weight and biomass ratio, were not affected by microparticles application. The highest yield of 6.82 kg m‑2 was obtained by application of T. viride spores and it was significantly higher than yield with the application of chitosan alginate microparticles with Ca2+ and Ca2+/T. viride. The application of T. viride resulted in significantly higher dry matter content of 7.07% compared to other treatments with determined values between 5.68 and 6.27% and control (6.20%). In comparison to the untreated control and treatment with T. viride spores, a significant increase of total chlorophylls (1986 µg g‑1 dw), polyphenols (4.58 mg EGK g‑1 dw) and flavonoids content (9.65 mg EK g‑1 dw), and antioxidant activity (21.92 µmol ET g‑1 dw) was recorded for the application of biopolymer microparticles containing the Ca2+. Based on the obtained results, the application of biopolymer microparticles could be recommended in lettuce open field growing to increase the phytochemicals content.
Benko, B., Haramija, F., Vinceković, M., Toth, N., Fabek Uher, S. and Žutić, I. (2021). Lettuce yield and nutritive value as affected by a biopolymer microparticles application. Acta Hortic. 1320, 181-188
biological and chemical agent, controlled release, encapsulation, Lactuca sativa