Somatic embryogenesis and organogenesis in some horticultural plants
Modern high-tech horticulture (fruit growing, floriculture, ornamental gardening and essential oil production) has been developed using advanced biotechnology methods for the improvement, propagation and conservation of plant species, cultivars and forms. In the Nikita Botanical Gardens, biotechnological researches have been carried out for many years and new methods of plant production are developed. The main principles of morphogenesis induction and morphogenetic capacity realization in vitro in a number of horticulture plants via somatic embryogenesis and organogenesis are determined. The effective protocols of somatic embryogenesis for ziziphus, common fig, clematis, caladium, lavandin, and other cultures have been improved and developed. High regeneration capacity was demonstrated in most fruits, ornamentals, and essential oil plants. The main inducers, triggers and inhibitors of cellular dedifferentiation and differentiation, callusogenesis, direct and indirect regeneration were revealed. As a part of the Global Strategy for Plant Conservation, a high-performance system for the long-term conservation of horticultural plants as in vitro collection of slow-growing plants was created on the base of the complex assessment of the morphometric, physiological and biochemical characteristics of the studied objects. It has been demonstrated that abiotic stressors had a stimulated effect on regenerative capacity increase in the subsequent plant culture under standard in vitro conditions and their adaptability in transfer from in vitro conditions to ex vitro and in vivo.
Mitrofanova, I.V. (2021). Somatic embryogenesis and organogenesis in some horticultural plants. Acta Hortic. 1324, 1-10
fruit, ornamental and essential oil plants, direct and indirect regeneration, slow-growing plant collection, in vitro