Structure of the leaf blades of some in vitro cultured horticultural plants

I.V. Mitrofanova, V.A. Brailko, S.V. Chelombit, T.I. Pilipchuk, N.N. Ivanova, O.V. Mitrofanova
Methods of plant tissue culture in vitro are widely used in modern biology. One of the main topics is investigation of in vitro plant assimilating organs structure, which is associated with their successful adaptation in vivo. Histological analyzes of the leaf blades in a number of valuable essential oil, fruit, and ornamental plants were carried out. Plants were cultured for 12 months on modified MS and PMb media in a climatic chamber at 24±1°C, 16-h photoperiod and light intensity at 37.5 μmol m‑2 s‑1. The leaf thickness varied from 91 to 235 μm. There was a thin cuticle on the epidermis surface (3-7 μm) in Rosa × damascene Mill., Rosa chinensis var. minima (Sims) Voss, Lavandula angustifolia Mill., Lavandula × intermedia Emeric ex Loisel., and Ficus carica L. Multiple simple and glandular trichomes (81-303 μm) on leaf blades surfaces in L. angustifolia, L. × intermedia, F. carica, Chrysanthemum × hortorum Bailey, and Diospyros kaki Thunb. were noted. Amphistomatic leaves were in Canna × hybrida hort. ex Backer and Ch. × hortorum, in the other species - hypostomatic. The number of stomatal apparatus mm‑2 varied from 15 to 265 ones. Mechanical and vascular tissues were developed in all the cultivars. Differentiated palisade and spongy tissues were characteristics of F. carica, R. × damascene, R. chinensis, L. anustustifolia, L. × intermedia, some D. kaki and Clematis L. cultivars. Palisade index varied from 0.32 to 0.58. Homogeneous mesophylls with large intercellular spaces were noted in C. × hybrida, most cultivars of D. kaki, Ch. × hortorum and Clematis. Thus, a number of cultures was identified which under in vitro propagation demonstrated structural features typical for ex situ plants, providing their better adaptation ex vitro.
Mitrofanova, I.V., Brailko, V.A., Chelombit, S.V., Pilipchuk, T.I., Ivanova, N.N. and Mitrofanova, O.V. (2021). Structure of the leaf blades of some in vitro cultured horticultural plants. Acta Hortic. 1324, 95-100
DOI: 10.17660/ActaHortic.2021.1324.14
horticultural plants, in vitro, anatomy, leaf blades, mesophyll

Acta Horticulturae