Biotechnological methods of maintaining collections of the genus Ribes L.

V.N. Sorokopudov, I.V. Knyazeva, O.A. Sorokopudova, J.V. Burmenko, T.V. Baranova
The development of effective methods for plant clonal micropropagation is a basic and necessary condition for creation of genebanks in vitro. Currently, the gene pool collection of samples of cultivated plants in vivo (Federal State Budgetary Scientific Institution All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery) genus Ribes L. has 295 cultivars: 137 – black currants, 45 – red currants, 82 – gooseberries, 31 – golden currants. Genetic collection in vitro of black currant includes the following cultivars: ‘Barmalej’, ‘Bryanskij’, ‘Agat’, ‘Gamayun’, ‘Kudesnik’, ‘Mif’, ‘Podarok’ ‘Veteranam’, ‘Strelec’, ‘Uslada’, ‘Charodej’ and ‘Yadrenaya’; golden currant – ‘Businka’. The deposition is carried out in the conditions of the cooling chamber (6±2°C), at 6-8 h photoperiod and light intensity of 500-1,000 lx. In vitro collection of currant is annually updating on two-three cultivars, free from malicious viruses: Arabis mosaic virus (ArMV), Raspberry ringspot virus (RpRSV), Tomato black ringspot virus (TBRV), Strawberry latent ringspot virus (SLRSV) and Raspberry bushy dwarf virus (RBDV). At the stage of explants, introduction MS culture medium was used with growth regulators, such as 6-benzylaminopurine (6-BAP), kinetin and zeatin in different concentrations (0.5, 1.0, 1.5 and 2 mg L‑1). The optimal variant was a medium with 6-BAP in a concentration of 0.5 mg L‑1 for black currant and 1.0 mg L‑1 for golden currant. The survival rate of black currant explants varied within 71.3-89.7%, golden currant – 37%. The multiplication rate among the black currant cultivars ranged from 1:2 (‘Gamayun’) to 1:4 (‘Bryanskij’, ‘Agat’), golden currant – 1:6. 6-BAP concentration increase to 2.0 mg L‑1 in culture medium increased the multiplication rate of golden currant to 1:8, but led to the appearance 35% hyperhydrated microshoots. The conservation of in vitro gene pool collection is cost-effective for the maintenance of biological diversity, but requires periodic testing for the genetic identity of the cultivar.
Sorokopudov, V.N., Knyazeva, I.V., Sorokopudova, O.A., Burmenko, J.V. and Baranova, T.V. (2021). Biotechnological methods of maintaining collections of the genus Ribes L.. Acta Hortic. 1324, 123-130
DOI: 10.17660/ActaHortic.2021.1324.19
Ribes, cultivar, in vitro collection, genetic resources

Acta Horticulturae