Ontomorphogenesis of axillary meristem-derived micropropagated plants of Rosa canina rootstock

O.Yu. Vasilyeva, E.V. Ambros, T.I. Novikova
Problem of garden roses reproduction in western Siberia is connected with the absence of seed plantations of winter-hardy rose-rootstocks. In Central Siberian Botanical Garden SB RAS (Novosibirsk) a comparative study of the plants' ontomorphogenesis of the selected highly winter-hardy Rosa canina form obtained by clonal micropropagation, seeds, and green cuttings was conducted in 2013-2018. Primary explants for in vitro multiplication were meristems with two leaf primordia, isolated from the axillary buds of annual shoots. The initiation of shoot formation was carried out in Murashige and Skoog (MS) liquid medium supplemented with 100.0 mg L‑1 glutathione, followed by cultivation on MS induction medium with 2.0 mg L‑1 benzyladenine (BA) and 1.0 mg L‑1 indole-3-acetic acid (IAA). At the micropropagation stage, the maximum number of microshoots explant‑1 (7.5±0.7) was registered on medium with 1.0 mg L‑1 BA. For rooting of the microshoots, 1/2 MS hormone-free medium supplemented with 1.0 mg L‑1 IAA was used. The regenerants were acclimatized in containers with sterile sand, followed by transferring into pots containing a peat with perlite, mold, sand, and coconut substrate. To date, in vivo conditions, the ontogenesis of R. canina of microclonal origin, including three ontogenetic states of the pregenerative period and two states of the generative period, has been described for the first time. In the selective form studied, all the shoot formation systems were preserved completely. They formed fruit shoots with 1-5 gipantia with full fruit-nuts. The technology of clonal micropropagation of selected forms of rootstock intended for seed plantations is effective for accelerated reproduction, since there is no need in stratification, and there is no vulnerable seedling in outdoor conditions. In addition, the in vitro technique used makes it possible regardless of the season, unlike green cuttings, which is important for conditions with a short vegetative period.
Vasilyeva, O.Yu., Ambros, E.V. and Novikova, T.I. (2021). Ontomorphogenesis of axillary meristem-derived micropropagated plants of Rosa canina rootstock. Acta Hortic. 1324, 143-150
DOI: 10.17660/ActaHortic.2021.1324.22
Rosa canina, rootstock, micropropagation, ontogenesis, western Siberia

Acta Horticulturae