Conservation and clonal micropropagation of rare endemic species Aristolochia manshuriensis
Aristolochia manshuriensis Kom. is a relict liana, endemic of the Manchurian floristic region with limited habitat, gradually shrinking due to anthropogenic impact. This species is listed in the Red Book of Russian Federation as an endangered one. A. manshuriensis hardly multiplies in generative and vegetative ways, so to obtain a sufficient amount of material for further reintroduction purpose, clonal micropropagation is used. For this species at the first stage, the influence of the explant age and its morphological features on the regeneration ability were investigated. As a result, it was found that the explants taken from young (not older than 4-6 years) plants of A. manshuriensis, were characterized by a higher ability to proliferate shoots compared with plants that have reached 12 years. The higher morphogenetic capacity was characterized by the explants taken from 2- to 3-year-old plants. The highest multiplication factor (14.84±0.8) was achieved on Murashige and Skoog medium with addition of 0.8 mg L‑1 BAP and 0.05 mg L‑1 IAA. A further increase of the concentration of BAP did not give a positive effect. The optimum culture medium for rooting was ½MS containing 3.0 mg L‑1 IBA. Adaptation was carried out using sphagnum moss and a mixture of sand, peat, and leaf-mold sod soil in a ratio of 1:1:1. Plants were grown at a temperature of 23-25°C, a 16-h photoperiod and an illumination of 3000 lx. It is shown that the micropropagation method in vitro can provide an opportunity for conservation and sustainable reproduction ex situ of the endangered endemic species, A. manshuriensis.
Molkanova, O.I., Gorbunov, Yu.N. and Egorova, D.A. (2021). Conservation and clonal micropropagation of rare endemic species Aristolochia manshuriensis. Acta Hortic. 1324, 19-26
rare plant species, in vitro tissue culture, ex situ preservation, apical and axillary bud explants, microshoots, rhizogenesis