Genetic diversity of Tulipa suaveolens Roth in the Crimea based on ISSR data

T. Kritskaya, A. Kashin
Inter simple sequence repeats (ISSR) analysis revealed significant polymorphism in Tulipa suaveolens Roth (= T. schrenkii Regel) populations in the Crimea and the adjacent territories of Russia. In terms of the number of polymorphic ISSR fragments, these populations far exceed the previously studied populations in the Lower and Middle Volga regions and the Orenburg oblast. The gene flow between the populations is 0.73 individuals per generation; and that figure is in accordance with the figures for other Tulipa species. The existence of gene flow and the hybrid nature of the majority of specimens are confirmed by the results obtained in NewHybrids software as well. The mountain population from the adjacencies of Yalta lacks any isolation which provides support for its inclusion in T. suaveolens species. The fact that, according to STRUCTURE, this population coalesces with the populations from the Rostov oblast provides evidence for Eugene Wulff's hypothesis (1930) that T. suaveolens got into the Crimea through the Isthmus of Perekop from the north. However, the obtained results do not dismiss the possibility that the species dispersal could have gone in the reversed direction. The pattern of the species' genetic polymorphism in the Crimea is further complicated by the fact that specimens from the Kerch populations form a discrete genetic group. Furthermore, there is a mixed population near the Koktebel town located between the mountain population near Yalta and the Kerch populations. This population is constituted of the specimens from both genetic groups. The genetic group that was identified by means of ISSR analysis in the specimens from the Kalmykia population was not the one that formed the structure of genetic polymorphism of the species' populations in the Crimea. There is evidence that the genetic group revealed for the specimens in the Markotkh population (Krasnodarsky region) was the one forming the structure of genetic polymorphism of the species, but, more likely, its contribution to the gene flow was not significant. In general, ISSR markers allow only for tentative conclusions. In order to draw more definitive conclusions, further analysis by means of the DNA sequencing method is required.
Kritskaya, T. and Kashin, A. (2021). Genetic diversity of Tulipa suaveolens Roth in the Crimea based on ISSR data. Acta Hortic. 1324, 297-304
DOI: 10.17660/ActaHortic.2021.1324.46
Tulipa suaveolens, Tulipa schrenkii, ISSR, Crimea

Acta Horticulturae