The use of high-throughput sequencing to investigate the diversity of defense protein genes of cucumber inoculated with Cucumber green mottle mosaic virus

E.A. Istomina, А.А. Shelenkov, A.A. Slavokhotova, T.V. Korostyleva, I.B. Korotseva, T.I. Odintsova
Cucumber green mottle mosaic virus (CGMMV) is an RNA-virus from the Tobamovirus genus that infects leaves and fruits of the plants from the Cucurbitaceae family, causing crop losses of up to 40%. The resistance genes to CGMMV have not been described so far, so attenuated strains, which do not cause disease symptoms, yet provide defense against pathogenic viral strains, are used for plant protection. CGMMV genome regions that determine pathogenicity were revealed earlier, but no studies were conducted to investigate differential expression of cucumber genes upon inoculation with pathogenic and attenuated viral strains in order to detect proteins involved in plant immune response, including antimicrobial peptides (AMP). In this work, such an investigation was performed by sequencing of eight cDNA libraries obtained from healthy, infected and vaccinated cucumber plants. The in silico search for plant defense proteins in the transcripts obtained revealed about 100 proteins involved in response to stress and pathogens in each of the samples. Differential expression analysis was performed for all such proteins, and significant differences in defense protein gene expression were revealed.
Istomina, E.A., Shelenkov, А.А., Slavokhotova, A.A., Korostyleva, T.V., Korotseva, I.B. and Odintsova, T.I. (2021). The use of high-throughput sequencing to investigate the diversity of defense protein genes of cucumber inoculated with Cucumber green mottle mosaic virus. Acta Hortic. 1324, 335-340
DOI: 10.17660/ActaHortic.2021.1324.51
https://doi.org/10.17660/ActaHortic.2021.1324.51
CGMMV, plant transcriptomes, differential gene expression, NGS
English

Acta Horticulturae