Genetic modification of jasmonate biosynthesis pathway in wheat alters plant tolerance to necrotrophic fungi

D. Miroshnichenko, A. Pigolev, A. Pushin, S. Dolgov, T. Savchenko
Jasmonates, phytohormones produced in allene oxide synthase branch of oxylipin biosynthesis pathway, regulate plant growth and development, flower formation, gene expression, fertility, photosynthesis, and stress responses. Formed in chloroplasts 12-oxophytodienoic acid (12-OPDA) and synthesized in peroxisomes jasmonic acid (JA) and JA derivatives are major metabolites of the pathway displaying biological activity. Studies performed on a model plant Arabidopsis thaliana demonstrate the involvement of jasmonates in the regulation of plant tolerance to necrotrophic phytopathogens. Here we report the analysis of transgenic wheat plants transformed with the Arabidopsis 12-OPDA REDUCTASE 3 (AtOPR3) gene, coding for peroxisome-targeted 12-oxo-phytodienoate reductase, one of the key enzymes of the jasmonate biosynthesis pathway. Analysis of the tolerance of independent transgenic lines to necrotrophic fungi Botrytis cinerea unexpectedly revealed their increased susceptibility to the pathogen. Interestingly, exogenous application of synthetic jasmonates on infected leaves also accelerates the disease symptoms development. Analysis of Arabidopsis plants with altered jasmonate content showed that, unlike in wheat, in Arabidopsis jasmonates improve tolerance to B. cinerea. Obtained results indicate that the jasmonate-regulated defense mechanisms differ between monocotyledonous wheat and dicotyledonous Arabidopsis.
Miroshnichenko, D., Pigolev, A., Pushin, A., Dolgov, S. and Savchenko, T. (2021). Genetic modification of jasmonate biosynthesis pathway in wheat alters plant tolerance to necrotrophic fungi. Acta Hortic. 1324, 341-346
DOI: 10.17660/ActaHortic.2021.1324.52
https://doi.org/10.17660/ActaHortic.2021.1324.52
genetic transformation, jasmonates, fungal phytopathogens, Botrytis cinerea
English

Acta Horticulturae