Adaptogenic effect of the Epin-extra drug on seed germination and seedlings growth of Cucumis sativus L. under cadmium stress

G.V. Reshetnik, S.I. Chmeleva, A.I. Sidyakin, S.F. Kotov
The purpose of our research was to study the influence of Epin-extra drug on catalase activity of germinating seeds and growth of seedlings in Сucumis sativus L. cultivar 'Fenix plus' under the effect of cadmium acetate at various concentrations. It was necessary to determine the optimal concentration of Epin-extra drug, to specify the pre-sowing seed treatment exposure, to ascertain the catalase activity of germinating seeds and the morphometric indicators of the seedlings. The solutions with different concentrations of cadmium acetate (10‑2-10‑4 М, 100 and 250 µM) were used. Epin-extra drug at the concentration of 0.05% as an optimal concentration was determined. The exposure of pre-sowing seed treatment was 4 h. Positive antistress influence of Epin-extra drug on the germination of С. sativus seeds under the effect of cadmium acetate was determined. Seed germination in solutions of cadmium acetate at various concentrations was inhibited from the first days of the experiment. At a salt concentration of 10‑2 М, the germination of the seeds was completely inhibited. At 10‑3 М, the germination rate was increased to 65%, at 10‑4 М - 73%. Pre-sowing seed treatment with Epin-extra drug increased this rate on 20%. The catalase enzyme activity was decreased due to increase of metal concentration in the medium. Pre-sowing seed treatment in 0.05% solution of Epin-extra drug stimulated activity of the catalase enzyme at maximum values of Cd(CH3COO)2 by an average on 10%. Under the effect of the metal during the vegetation, the indicators of growth of the aerial part and the root system of the cucumber seedlings were below the test ones by an average on 40%. The exogenous epibrassinolide was increased of the aerial part on 15%, the wet weight on 17% and the dry weight on 29%. As a result, the studies have shown that the pre-sowing treatment of С. sativus seeds with the growth regulator Epin-extra induces the resistance to sublethal concentrations of cadmium, contributing to decrease of the inhibitory effects of the growth processes.
Reshetnik, G.V., Chmeleva, S.I., Sidyakin, A.I. and Kotov, S.F. (2021). Adaptogenic effect of the Epin-extra drug on seed germination and seedlings growth of Cucumis sativus L. under cadmium stress. Acta Hortic. 1324, 443-449
DOI: 10.17660/ActaHortic.2021.1324.68
https://doi.org/10.17660/ActaHortic.2021.1324.68
Epin-extra drug, cadmium, catalase, morphometric indicators
English

Acta Horticulturae