Aconitum lasiostomum Reichenb. ex Bess. is a promising plant for biotechnology research

I.V. Mitrofanova, N.P. Lesnikova-Sedoshenko, A.E. Paliy, O.V. Startseva, M.I. Rudenko, S.V. Chelombit, O.V. Mitrofanova
Aconitum lasiostomum Reichenb. ex Bess. (genus Aconitum, family Ranunculaceae) is one of the most rare species in the Crimea. It is a perennial herb, an endangered species of the western Palearctic region. By its ecological nature, the species is shade tolerant, petrophyte, and mesophyte one. In the Crimea, it grows in the upper part of the northern macroslope of the Main Ridge of the Crimean Mountains at an altitude 1,000-1,500 m a.s.l. The species has been listed in the Red Data Book of the Russian Federation and regional Red Books of the European part of Russia and some eastern European countries. Despite the fact that all parts of this plant are poisonous, A. lasiostomum is of great interest and is used in folk medicine. In recent years, its population decreased, mainly due to uncontrolled collecting of plants or their parts as medicinal raw materials. Biotechnological researches on A. lasiostomum in vitro propagation and preservation have been first started in the Nikita Botanical Gardens. The objects of the research were native samples (leaves, inflorescences, seeds, and fruits) of A. lasiostomum collected in July-September for biochemical analyses and introduction in vitro. The total content of phenolic substances in the aconite inflorescences was 1,742 mg 100 g‑1 of fresh biomass, in leaves NDASH 2,241 mg 100 g‑1, in fruits NDASH 1,608 mg 100 g‑1. Further studies on A. lasiostomum flavonoid identification are of a great importance as far as Aconitum species are promising sources of this class of substances. Optimal conditions for in vitro culture were revealed and A. lasiostomum plantlets and adventitious microshoots, which can be used for further studies of regeneration capacity, biochemical analysis for the content of biological active substances, micropropagation, and conservation of this species, were obtained on MS culture medium supplemented with BAP, IBA, and GA3.
Mitrofanova, I.V., Lesnikova-Sedoshenko, N.P., Paliy, A.E., Startseva, O.V., Rudenko, M.I., Chelombit, S.V. and Mitrofanova, O.V. (2021). Aconitum lasiostomum Reichenb. ex Bess. is a promising plant for biotechnology research. Acta Hortic. 1324, 47-54
DOI: 10.17660/ActaHortic.2021.1324.7
rare species, regeneration capacity, in vitro, phenolic substances, flavonoids

Acta Horticulturae