Single-spore isolation protocol for characterization of postharvest pathogens causing bitter rot of apple in South Tyrol
South Tyrol (northern Italy) is the largest continuous apple-producing area in Europe. Apples can be stored for prolonged periods due to advanced conservation technologies. Nevertheless, pathogenic fungi can induce post-harvest diseases during and after storage and lead to significant losses. Bull's eye rot and bitter rot, which comprise several species of the genera Neofabraea and Colletotrichum, represent important diseases of apple fruit both during cultivation as well as during storage. These fungi are difficult to be distinguished based merely on symptoms on the fruit and usually require expert phytopathological support for determination, including molecular analysis. Reliable identification of specific pathogens, however, is the basic requirement for studying various aspects of post-harvest diseases, as well as for the development of effective strategies for disease management. The present study describes a protocol for obtaining single-spore isolates of the genus Colletotrichum, causing bitter rot of apple. Pure cultures obtained by the here described procedure can be used for the investigation of morphological characteristics by employing microbiological and microscopic procedures, as well as to apply or develop novel diagnostic procedures based on nucleic-acid analysis or on the application of MALDI- TOF (Matrix Assisted Laser Desorption Ionization - Time of Flight) mass spectrometry.
Amaral Carneiro, G. and Baric, S. (2021). Single-spore isolation protocol for characterization of postharvest pathogens causing bitter rot of apple in South Tyrol. Acta Hortic. 1325, 1-6
Malus × domestica, Colletotrichum spp., fungal sporulation, single-spore culture, diagnostic methods