The effects of AM fungi and nitrogen fixation bacteria on growth and root morphology parameters of pea (Pisum sativum) plants
The study aimed to investigate the effects of a commercially available AMF mixture (Glomus intraradices, Glomus etunicatum, Glomus mosseae, Glomus geosporum, and Glomus clarum) and nitrogen fixation bacteria (Rhizobium leguminosarum and Burkholderia spp.), either supplied individually or in combination with each other, on the growth and root morphology parameters of field pea (Pisum sativum L.) plants. Inoculated and non-inoculated pea plants were subjected to three levels of salinity by the addition of sodium chlorate into the tap water (0, 20 and 50 mM NaCl). Several times during the growing cycle, in randomly selected plants the morphology of root system was analyzed and dry matter of roots and shoots were individually measured. The raise of salinity adversely affected the growth of pea plants. Root dry matter and shoot dry matter were gradually decreased and almost all root morphology parameters (root length, root projected area, root surface area, root volume) were also deteriorated. The inoculations of either AMF or bacteria were followed by a significant increase of root and shoot dry matter in AMF or bacteria inoculated versus the control plants. That was found statistically significant, in both; the non-saline (0 mM NaCl) and saline (20 and 50 mM NaCl) conditions. The presence of AM fungi and bacteria significantly increased root length as well, root projected area, root surface area and root volume under moderate salinity conditions. Surprisingly, the combined use of AM fungi with either Rhizobium or Burkholderia, did not show any synergetic growth enhancement effect either regarding dry matter or root morphology parameters.
Veselaj, E., Sallaku, G. and Balliu, A. (2021). The effects of AM fungi and nitrogen fixation bacteria on growth and root morphology parameters of pea (Pisum sativum) plants. Acta Hortic. 1326, 295-302
AMF, Rhizobia, Burkholderia, dry matter, root length, root surface area, root volume