Identification and application of sex-linked markers in kiwifruit

Q. Zhang, Y.F. Liu, S.B. Wang, H. Xiong, H. Zheng, X.H. Yao, Z.P. Wang, C.H. Zhong
Kiwifruit (Actinidia chinensis Planchon) is an important specialty fruit crop with high nutritional value. Kiwifruit is functionally dioecious, which presents unique challenges for breeding and commercial production. In this study, putative sex-related loci within a previously determined sex-related chromosome were identified through genotyping-by-sequencing (GBS) of 5 female and 5 male kiwifruit samples. Sex-specific polymorphisms were identified, and a total of 352 male specific SNPs were detected in all male samples. Among those male specific polymorphisms, 8 SNPs were found to map to chromosome 25, the sex determination region for A. chinensis. Sex-related markers were developed for these 8 candidate loci, and 2 show promise in which can distinguishing male and female kiwifruit plants. By providing a means for sex typing in A. chinensis kiwifruit, sex-linked SNP markers have the potential to facilitate high-throughput sex screening of seedling populations.
Zhang, Q., Liu, Y.F., Wang, S.B., Xiong, H., Zheng, H., Yao, X.H., Wang, Z.P. and Zhong, C.H. (2022). Identification and application of sex-linked markers in kiwifruit. Acta Hortic. 1332, 65-70
DOI: 10.17660/ActaHortic.2022.1332.9
kiwifruit, sex determination, GBS, sex-linked marker, sex screening

Acta Horticulturae