In vitro propagation of Artocarpus lakoocha Roxb.: effect of meta-topolin on shoot parameters

This study aimed to improve in vitro propagation and conservation options for Artocarpus lakoocha Roxb. (lakoocha), a multipurpose tree species native to India.
For in vitro propagation, the plant growth hormones, benzylaminopurine (BAP) and meta-topolin (MT) were used.
MS medium was supplemented with different concentrations of BAP (0, 0.2, 0.5, 1, 2, and 5 mg L^‑1), MT (0, 0.5, 1, 2, and 5 mg L^‑1) and the shoot parameters were recorded.
The results showed that BAP was better for shoot multiplication than MT. There was no significant difference observed in number of nodes by various concentrations of BAP and MT. As compared to control, BAP and MT showed much improvement on all shoot parameters.
Callus was observed at higher concentrations of both BAP and MT. BAP at 0.5 mg L^‑1 adjudged the best.
On this medium, the average number of shoots, shoot length and number of nodes were 5.42±1.80, 2.25±0.23 cm, and 2.48±0.18, respectively, after six months.

IX International Scientific and Practical Conference on Biotechnology as an Instrument for Plant Biodiversity Conservation (physiological, biochemical, embryological, genetic and legal aspects)

S. Gupta, V.K. Biraji

monkey jack, lakoocha, Artocarpus spp., meta-topolin, tissue culture

https://doi.org/10.17660/ActaHortic.2022.1339.2